Establishment of reverse-phase high-performance liquid chromatography with chiral reagent derivatization for separation of fexofenadine enantiomers.
- Author:
Qing-qing YAO
1
;
Bo-xuan QU
1
;
Quan ZHOU
1
;
Su ZENG
1
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Stereoisomerism; Terfenadine; analogs & derivatives; analysis
- From: Journal of Zhejiang University. Medical sciences 2014;43(2):155-159
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a precolumn chiral derivatization method for determination of fexofenadine enantiomers, a chiral substrate of OATP1B1, in cellular model.
METHODSR-(+)-phenylethyl isocyanate was selected as chiral derivatization reagent, which was reacted with fexofenadine to form carbamate derivatives. Enantiomers were identified by LC/MS and separated by RP-HPLC.
RESULTSUnder the experimental conditions, the fexofenadine enantiomers were separated completely. The standard curve was linear over the concentration range of 25-100 ng/ml (R(2)=0.9992, 0.9989). Accuracy was 101.1% and 98.3%, intra-precision was 2.4% and 3.1%, inter-precision was 3.1% and 4.0% for D1 and D2, respectively.
CONCLUSIONThe method established is sensitive and accurate for determination of fexofenadine enantiomers in cells.
