Purification of polyphenols from Sabina vulgaris antoine and its antioxidant properties.

Long LI; Gui-da XUAN; Ping CHEN

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Purification of polyphenols from Sabina vulgaris antoine and its antioxidant properties.

Author: Long LI ^{1
,

2} ; Gui-da XUAN ³ ; Ping CHEN ⁴
Author Information

1. School of Medicine, Zhejiang University City College, Hangzhou 310015,China;
2. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310018, China;
3. School of Medicine, Zhejiang University City College, Hangzhou 310015,China.
4. College of Science, Zhejiang University, Hangzhou 310018, China.
Publication Type:Journal Article
MeSH: Antioxidants; pharmacology; Cupressaceae; chemistry; Polyphenols; isolation & purification
From: Journal of Zhejiang University. Medical sciences 2014;43(2):175-179
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo purify polyphenols from Sabina vulgaris and to investigate its antioxidant properties.
METHODSPolyphenols were purified from Sabina vulgaris Antoine with macroporous resin HPD-700, and the quantity of polyphenols was determined by Folin-Ciocalteu colorimetry. The antioxidant properties of polyphenols were evaluated by total antioxidant capacity (T-AOC) and its activities of scavenging DPPH (1,1 diphenyl-2-picry-hydrazyl) radicals, superoxide anion (O2·-), hydroxyl free radicals (OH·) and ferric reducing antioxidant power (FRAP).
RESULTSAfter purification, the purity of polyphenols increased from 0.053% to 0.995%.The antioxidant properties study showed that its inhibition rate of scavenging DPPH radicals and FRAP was 151.83 U/ml and 204.59 U/ml. Its scavenging capacity for superoxide anion (O2·-) and hydroxyl free radicals (OH·) was 151.83 U/ml and 204.59 U/ml. The total antioxidant capacity was 72.68 U/ml.
CONCLUSIONPolyphenols from Sabina vulgaris Antoine have high antioxidant properties, suggesting that it worth further study of its pharmacological effects.