c-SRC knockdown decreases phosphorylated STAT3 expression and viability of HeLa cells.
- Author:
Jia-Xiang CHEN
1
;
Lin-Lin XU
;
Sheng-Jiao WU
;
Hong-Yu LIU
;
Jing-Lei WANG
;
Ting ZOU
Author Information
1. Department of Physiology, Nanchang University, Nanchang, China.
- Publication Type:Journal Article
- MeSH:
Cell Survival;
Female;
Gene Expression Regulation, Neoplastic;
Gene Knockdown Techniques;
Genes, src;
genetics;
HeLa Cells;
Humans;
Phosphorylation;
RNA, Messenger;
biosynthesis;
genetics;
STAT3 Transcription Factor;
genetics;
metabolism;
Transfection
- From:
Acta Physiologica Sinica
2011;63(3):198-204
- CountryChina
- Language:Chinese
-
Abstract:
The present study was to determine the effect of c-SRC on the viability of human cervical cancer HeLa cells and the expression of phosphorylated signal transducer and activator of transcription-3 (p-STAT3) of the cell. Post-transfection of c-SRC RNA interference vector, RT-PCR and Western blot were utilized to observe the contents of c-SRC mRNA and protein, respectively, in HeLa cells. The MTT was used to observe the viability of the cells. Cell cycle was observed by flow cytometry. The content of p-STAT3 in the cells was also investigated after knockdown of c-SRC. Knockdown of c-SRC significantly decreased the contents of c-SRC mRNA and protein in the cells. The viability of the cells decreased by 23.1%, 29.3%, 38.6% and 45.0% (all P < 0.05), respectively, after the cells were transfected with c-SRC RNA interference vector for 24, 48, 72, and 96 h. The number of S-phase cells decreased by 5.6%, 10.0%, 15.2% and 19.9% (all P < 0.05), respectively, after transfection of c-SRC RNA interference vector for 24, 48, 72, and 96 h. The content of p-STAT3 also decreased when c-SRC was knockdowned. Compared with the control group, after treatment of HeLa cells with STAT3 inhibitor Piceatannol for 24, 48, 72, and 96 h, the cell viability decreased by 23.8%, 29.7%, 37.3% and 45.4% (all P < 0.05), respectively, while increase of c-SRC content could not reverse the inhibitory effect. These results suggest that the inhibited viability of HeLa cells caused by knockdown of c-SRC is associated with the decreased content of p-STAT3 protein.
