Effect of resveratrol on lipopolysaccharide-induced activation of rat peritoneal macrophages.
- Author:
Zhen-hua MA
1
;
Qing-yong MA
;
Huan-chen SHA
;
Lian-cai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cytokines; metabolism; Lipopolysaccharides; pharmacology; Macrophage Activation; drug effects; Macrophages, Peritoneal; drug effects; immunology; metabolism; Male; NF-kappa B; metabolism; Nitric Oxide; metabolism; Rats; Rats, Sprague-Dawley; Stilbenes; pharmacology
- From: Journal of Southern Medical University 2006;26(9):1363-1365
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate nuclear factor kappa B (NF-kappaB) activation induced by lipopolysaccharide (LPS) in rat peritoneal macrophages (PMAs) and the inhibitory effect of resveratrol on NF-kappaB activation.
METHODSPMAS from normal SD rats were randomly divided into 7 groups, including a control group, a LPS group and 5 resveratrol groups (I-V). PMAs of the control group were incubated in DMEM, and those in LPS group in DMEM containing LPS (10 microg/ml). PMAS of resveratrol groups I-V were incubated in DMEM containing LPS (10 microg/ml) and different concentrations of resveratrol. After 24 h of incubation, NF-kappaB activation in the PMAs was determined, and the expression levels of tumor necrosis factor alpha (TNF-alpha), interleukin-1 (IL-1) and nitric oxide (NO) in the culture medium were measured.
RESULTSExposure to LPS resulted in an excessive enhancement of cytokine and NO expressions in the PMAs. Resveratrol at 1.25-10 microg/ml produced a dose- dependent inhibition of cytokine and NO expressions and on NF-kappaB activation in LPS-stimulated PMAs.
CONCLUSIONResveratrol can inhibit LPS-induced NF-kappaB activation in rat PMAs and subsequently suppress the expressions of TNF-alpha, IL-1 and NO.
