Study on the DNA immunogenicity of fusion and attachment glycoproteins of Nipah virus.
- Author:
Xi-Jun WANG
1
;
Jin-Ying GE
;
Qing-Hua WANG
;
Sen HU
;
Xiang-Mei LIN
;
Zhi-Gao BU
Author Information
1. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Viral;
blood;
Blotting, Western;
Enzyme-Linked Immunosorbent Assay;
Female;
Mice;
Mice, Inbred BALB C;
Nipah Virus;
immunology;
Vaccines, DNA;
immunology;
Viral Envelope Proteins;
genetics;
immunology;
Viral Vaccines;
immunology
- From:
Chinese Journal of Virology
2008;24(1):47-52
- CountryChina
- Language:Chinese
-
Abstract:
The two mammalian codon optimized genes, F and G genes of Nipah virus, were generated by assembly PCR, and inserted into mammalian expression vector pCAGGS under chicken beta-actin promoter to construct pCAGG-NiV-F and pCAGG-NiV-G. Syncytium formation was induced in BHK cells by plasmid pCAGG-NiV-F and pCAGG-NiV-G transfection, which indicate recombination proteins F and G were expressed in BHK cell and possessed good biologic activity. Six-week-old female BALB/c mice were intramuscularly primed with 100 microg pCAGG-NiV-F, pCAGG-NiV-G or pCAGG-NiV-F+ pCAGG-NiV-G respectively, and boosted with same dose after 4 weeks. The sera were collected at 3 weeks post second boost. The serum IgG against Nipah virus F and G proteins was detected by indirect ELISA using recombinant Baculovirus expressed Nipah F and G glycoproteins. The results showed that specific antibodies possessed good sensitivity and specificity. Furthermore, the G and F proteins' specific antibodies could neutralize the infectivity of VSVdeltaG* F/G (the NiV F and G envelope glycoproteins psudotyped recombinant vesicular stomatitis virus expressing green fluorescence protein). And, pCAGG-NiV-G also induced higher titer of neutralizing antibody response than pCAGG-NiV-F did. The result indicates that DAN immunization is an efficient vaccine strategy against Nipah virus.
