Inhibition of thermal hyperalgesia and tactile allodynia by intrathecal administration of gamma-aminobutyric acid transporter-1 inhibitor NO-711 in rats with chronic constriction injury.
- Author:
Shan-Shan ZHU
1
;
Yin-Ming ZENG
;
Jun-Ke WANG
;
Rong YAN
;
Xin NIE
;
Jun-Li CAO
Author Information
1. Department of Anesthesiology, the First Affiliated Hospital, China Medical University, Shenyang 110001, China. xzzss2004@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
GABA Antagonists;
administration & dosage;
pharmacology;
Hyperalgesia;
drug therapy;
physiopathology;
Injections, Spinal;
Male;
Neurotransmitter Uptake Inhibitors;
administration & dosage;
pharmacokinetics;
Nipecotic Acids;
administration & dosage;
pharmacology;
Oximes;
administration & dosage;
pharmacology;
Pain;
physiopathology;
Rats;
Rats, Sprague-Dawley;
Sciatic Neuropathy;
drug therapy;
physiopathology
- From:
Acta Physiologica Sinica
2005;57(2):233-239
- CountryChina
- Language:English
-
Abstract:
The present study was undertaken to explore the role of gamma-aminobutyric acid transporters in the neuropathic pain. On the chronic constriction injury (CCI) rats 4 doses (5, 10, 20, 40 microg in group N5, N10, N20, N40, respectively) of specific gamma-aminobutyric acid transporter-1 inhibitor NO-711 or normal saline (in group NS) were intrathecally administered before sciatic nerve ligation (pre-treatment) or at the third day after ligation (post-treatment). The paw withdrawl latency (PWL) from a noxious thermal stimulus and paw withdrawl mechanical threshold (PWMT) of von Frey filament was used as measure of thermal hyperalgesia and tactile allodynia respectively. The results demonstrated that post-treatment of NO-711 significantly suppressed thermal hyperalgesia and allodynia in CCI rats (P<0.05, P<0.01), the inhibitory effect lasted for 2 h (N40 group) and 4 h (N20 group) respectively. NO-711 inhibited thermal hyperalgesia induced by CCI in a dose-dependent manner. Intrathecal pretreatment with different doses of NO-711 delayed the occurrence of thermal hyperalgesia, but could not delay the emergence of allodynia induced by CCI. This study indicates that gamma-aminobutyric acid transporter inhibitor has anti-thermal hyperalgesia and anti-tactile allodynia effects in neuropathic rats.
