Isolation and identification of arboviruses from mosquito pools in Yunnan Province.
- Author:
Xiao-hong SUN
1
;
Shi-hong FU
;
Hai-lin ZHANG
;
Huan-yu WANG
;
Ying HE
;
Wei-bin LIU
;
Wei-hong YANG
;
Yun FENG
;
Ji-guang MIN
;
Rui-hong HAN
;
Guo-dong LIANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Arboviruses; classification; genetics; isolation & purification; Cell Line; China; Cricetinae; Culicidae; virology; Encephalitis Virus, Japanese; classification; genetics; isolation & purification; Encephalitis, Japanese; virology; Genotype; Microscopy, Fluorescence; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; methods; Sequence Analysis, DNA; Viral Envelope Proteins; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2005;19(4):319-324
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo survey arboviruses in Yunnan province.
METHODSMosquitoes were collected from Yunnan Province in 2002 and 2004. Virus strains were isolated by the inoculation of homogenates of the mosquitoes onto BHK cell line. The isolated strains and their molecular biological characteristics were identified by real-time PCR, reverse transcription-polymerase chain reaction (RT-PCR) and immunofluorescent antibody technique.
RESULTSTwelve strains of viruses producing CPE in BHK cells were isolated from 4810 mosquitoes. All the 12 isolates were identified to be Japanese encephalitis viruses. Genotype analysis showed the new virus (DL-0437 strain) belonged to genotype III.
CONCLUSIONTwelve strains of Japanese encephalitis viruses were isolated from mosquito pools collected in Yunnan. It was the first isolation of genotype III Japanese encephalitis viruses in Yunnan Province in recent years.
