Experimental study on He-Ne laser irradiation to inhibit scar fibroblast growth in culture.
- Author:
Bin SHU
1
;
Zongyao WU
;
Linlin HAO
;
Dengfen ZENG
;
Guangrui FENG
;
Yonghui LIN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; radiation effects; Cells, Cultured; Cicatrix; pathology; Dose-Response Relationship, Radiation; Female; Fibroblasts; cytology; radiation effects; Helium; Humans; Lasers; Male; Neon
- From: Chinese Journal of Traumatology 2002;5(4):246-249
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo explore the inhibitory effect of He-Ne laser irradiation on fibroblast growth of hypertrophic scars in culture.
METHODSHe-Ne laser with wavelength of 632.8 nm, power density of 50 mW/cm(2) and doses of 3 J/cm(2), 30 J/cm(2), 90 J/cm(2) and 180 J/cm(2) was used to irradiate human scar fibroblasts in culture 1, 3 and 5 times respectively, and then the cell count and cell cycle analysis were done.
RESULTSRepeated irradiation with He-Ne laser at dose of 180 J/cm(2) three and five times led to an evident decrease in total cell number compared with that of the control group and there was a significant difference (P<0.05). The cell cycle analysis showed after three and five times of irradiation with 180 J/cm(2) He-Ne laser the cell number in S-phase decreased from 51% to 20% and 14% respectively, the cell number in G(0)/G(1) phase increased from 28% to 55% and 60% respectively, and the cell percentage in Sub-G1 phase was 6.7% and 9.8% respectively.
CONCLUSIONSRepeated irradiation with 180 J/cm(2) He-Ne laser can inhibit scar fibroblasts growth in culture. It may be that He-Ne laser irradiation causes cell stagnation in G(0)/G(1) phase and apoptosis.
