Mitochondrial mechanisms of apoptosis of human leukemia K562 cells induced by AVVC-1.
10.7534/j.issn.1009-2137.2013.03.011
- Author:
Ru-Qi ZHENG
1
;
Gen-Bao ZHANG
;
Lu HUANG
;
Kai-Ran MA
;
Juan WU
;
Shu LI
Author Information
1. Department of Pathophysioloy, Wannan Medical College, Wuhu, Anhui Province, China.
- Publication Type:Journal Article
- MeSH:
Agkistrodon;
Animals;
Apoptosis;
drug effects;
Cytochromes c;
metabolism;
Humans;
K562 Cells;
Membrane Potential, Mitochondrial;
drug effects;
Mitochondria;
metabolism;
Snake Venoms;
pharmacology
- From:
Journal of Experimental Hematology
2013;21(3):591-595
- CountryChina
- Language:Chinese
-
Abstract:
This study was purpose to investigate apoptosis pathway of leukemia K562 cells induced by anticoagulant fraction from Agkistrodon acutus venom (AVVC-1). The mitochondrial transmembrane potential (ΔΨm) of leukemia K562 cells was detected by flow cytometry with JC-1 single staining. The expression of cytochrome C in the mitochondrial of leukemia K562 cells was analyzed by Western blot after AVVC-1 treatment. The distribution of cytochrome C in leukemia K562 cells was measured by immuno-fluorescence test. The results showed that the potential of mitochondrial membrane decreased after treatment with different concentrations of AVVC-1 (12.5, 25, 50, 100 µg/ml) for 6 h (P < 0.01). The expression level of cytochrome C protein in mitochondria obviously declined after treatment with 30 µg/ml AVVC-1 for 48 h, and the fluorescent intensity of cytochrome C in cytosol was enhanced at the same time. It is concluded that AVVC-1-induced K562 cell apoptosis is related with mitochondrial damage, and cytochrome C may be a useful agent for investigating human leukemia therapy by using AVVC-1.
