Analysis of beta-lactams-resistance genes in Pseudomonas aeruginosa in burn ward.
- Author:
Qi-Fa SONG
1
;
Jian ZHENG
;
Hui LIN
;
Jing-Ye XU
;
Chun-Guang JIN
;
Guo-Jun LI
Author Information
- Publication Type:Journal Article
- MeSH: Burn Units; Burns; microbiology; Genes, Bacterial; Humans; Pseudomonas aeruginosa; drug effects; genetics; isolation & purification; beta-Lactam Resistance; genetics
- From: Chinese Journal of Burns 2007;23(3):212-215
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the resistance genes and antibiotic resistance patterns against beta-lactams in Pseudomonas aeruginosa prevalent in burn ward.
METHODSK-B method was performed to test bacterial resistance patterns against 9 species of beta-lactams in Pseudomonas aeruginosa isolated from wounds and dressings of the patient in burn wards. Seven species of resistance genes against beta-lactams were detected with PCR. Tazobactam-inhibited piperacillin resistance test was performed to study whether the above strains produce extended spectrum beta-lactams.
RESULTSAll 12 strains of bacteria with resistance genes detected were resistant to penicillin and cephalosporins (100%), among them 11 were resistant to all antibiotics. Tazobactam-inhibited piperacillin resistance test demonstrated that all strains with resistance genes were ESBLs.
CONCLUSIONHigh incidence of beta-lactams resistance genes is found in Pseudomonas aeruginosa isolated from burn ward, and they have close relationship with the occurrence of multiple drug-resistance.
