Fluorescence spectra and test application study on the interaction of fluorescein and bovine serum albumin.
- Author:
Xun HUANG
1
,
2
;
Ai'ming SUN
;
Xueqin DING
;
Xiucheng XU
;
Luyan FANG
Author Information
1. Department of Biomedical Analysis, Analysis and Testing Center, Sichuan University, Chengdu 610041, China. huangfxcz@
2. com
- Publication Type:Journal Article
- MeSH:
Animals;
Cattle;
Drug Interactions;
Fluorescein;
chemistry;
Serum Albumin, Bovine;
analysis;
Spectrometry, Fluorescence;
methods
- From:
Journal of Biomedical Engineering
2006;23(6):1325-1327
- CountryChina
- Language:Chinese
-
Abstract:
A fluorescence method is found for determination of bovine serum albumin (BSA). The method is based on the interaction of fluorescein with BSA to form a complex in Tris-HCl buffer of pH 2.2. The complex exitation wavelength is 467 nm, the emission wavelength is 515 nm. The linear measurement rang is between 1.8-500 mg/L for BSA, F = 26.776 C + 2.8082, r = 0.9999. This method is sensitive,steady,and low cost for determination of BSA.
