Effects of AZT on leukemia cell line KG-1a proliferation and telomerase activity.
- Author:
Rui-Rui JIN
1
;
Rong CHAO
;
Ya-Ming XI
;
Che CHEN
;
Hui-Yuan CHU
;
Ming LI
;
Hao ZHANG
Author Information
1. Department of Hematology, The First Clinical Hospital of Lanzhou University, Lanzhou, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
drug effects;
Cell Cycle;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Humans;
Leukemia;
metabolism;
pathology;
Telomerase;
antagonists & inhibitors;
metabolism;
Zidovudine;
pharmacology
- From:
Journal of Experimental Hematology
2012;20(2):277-281
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the effect of 3'-azido-2', 3'-dideoxythymidine (AZT)on the proliferation and telomerase activity of human acute myeloid leukemia cell line KG-1a. The effect of proliferation was detected by MTT assay after the KG-1a cell were stimulated for 24, 48 and 72 h with different concentrations of AZT; telomerase activity was detected with TRAP-PCR-ELISA assay; RT-PCR was used to detect telomerase hTERT mRNA expression. The results showed that the proliferation of KG-1a cells was inhibited in a time and concentration dependent manner after exposure to AZT for 24, 48 and 72 h; the KG-1a cells decreased in S phase and increased in G(2)/M phase with the increasing of the concentration of AZT; telomerase activity and hTERT-mRNA expression in the experimental groups decreased after treated with AZT, which was positively correlated with concentration of AZT. It is concluded that AZT inhibits KG-1a cell proliferation and induces apoptosis, which maybe related with its decreasing the telomerase activity and hTERT mRNA expression.
