Synthesis of CCK-8 tetrapeptide fragment by enzymatic method.
- Author:
Guangya XIANG
1
;
Heiner ECKSTEIN
Author Information
1. Pharmacy School, Tongji Medical College, Huazhong University of Science and Technology, Wuhan
- Publication Type:Journal Article
- MeSH:
Catalysis;
Chymotrypsin;
Oligopeptides;
Papain;
Peptide Fragments;
Sincalide;
chemical synthesis
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2003;23(3):234-241
- CountryChina
- Language:English
-
Abstract:
The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported. This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEt successively. These three steps were catalyzed by alpha-chymotrpsin, Papain and alpha-chymotrpsin respectively. The results of FAB-MS showed that all the products had the correct molecular mass.
