Molecular identification of hairy antler by analysis of high resolution melting.
- Author:
Kang CHEN
;
Chao JIANG
;
Yuan YUAN
;
Lu-qi HUANG
;
Yan JIN
- Publication Type:Journal Article
- MeSH:
Animals;
Antlers;
chemistry;
DNA;
chemistry;
genetics;
DNA Primers;
genetics;
Deer;
classification;
genetics;
Genotype;
Medicine, Chinese Traditional;
standards;
Polymerase Chain Reaction;
Transition Temperature
- From:
China Journal of Chinese Materia Medica
2015;40(4):619-623
- CountryChina
- Language:Chinese
-
Abstract:
High resolution melting (HRM) , an important technology for genotyping and mutation scanning, has broad prospects in the authenticity of traditional Chinese medicine. This paper selected universal CO I primers and used HRM to establish a new method for authenticity of Hairy Antler. PCR was conducted at the annealing temperature of 60 °C and 45 cycles. The range of the DNA template concentration, the primer concentration and the Mg2+ ion concentration were further optimized. The results showed that the Tm values of Cervus nippon were (81.96 ± 0.07), (84.51 ± 0.03) °C and Cervus elaphus was(82.58 ± 0.13), (85.95 ± 0.05) °C with 10-100 mg · L(-1) DNA template, 0.2 µLmol · L(-1) primer, 2.0 mmol · L(-1) Mg2+. This method can authenticate of hairy antler and is simple, fast, high-throughput, visualization.
