Effect of artificial musk aqueous extract on the expressions of inflammatory mediators released from lipopolysaccharide-stimulated RAW264.7 cells.
- Author:
Yu MENG
1
;
Qin-Wen ZOU
1
;
Jin-Ye BAI
2
;
Gui-Fang CHENG
2
;
Xuan XIAO
2
;
Shu ZHANG
1
;
Xiu-Yuan ZHU
2
;
Xiao-Liang WANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents; pharmacology; Cell Line, Tumor; Cytokines; metabolism; Enzyme-Linked Immunosorbent Assay; Fatty Acids, Monounsaturated; pharmacology; Inflammation Mediators; metabolism; Interleukin-6; metabolism; Lipopolysaccharides; Macrophages; Tumor Necrosis Factor-alpha; metabolism
- From: Acta Academiae Medicinae Sinicae 2014;36(6):583-586
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo identify the anti-inflammatory effects of artificial musk aqueous extract(AME)on lipopolysaccharide-stimulated cytokines secreted or released by RAW264.7 cells.
METHODSCytokines including interleukin(IL)-6,IL-10,and tumor necrosis factor Α were determined using cytokine enzyme-linked immunosorbent assay kits.
RESULTCompared with model group,the levels of major cytokines such as tumor necrosis factor Α,IL-6,and IL-10 significantly decreased in different AME groups in a dose-dependent manner.
CONCLUSIONIn lipopolysaccharide-stimulated RAW264.7 macrophages,AME can remarkably inhibit the release of inflammatory cytokines and thus exerts its anti-inflammatory effects.
