Chitosan/PIK3CA siRNA nanoparticle-mediated PIK3CA gene interference decreases the invasive capacity of gastric cancer cells in vitro.
- Author:
Xinke ZHOU
1
,
2
;
Lu HE
;
Min LIANG
;
Jifang LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cell Proliferation; Chitosan; Class I Phosphatidylinositol 3-Kinases; Humans; Nanoparticles; Phosphatidylinositol 3-Kinases; genetics; RNA, Small Interfering; Real-Time Polymerase Chain Reaction; Stomach Neoplasms; pathology
- From: Journal of Southern Medical University 2014;34(10):1503-1506
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of PIK3CA/siRNA chitosan nanoparticle on the invasiveness of gastric carcinoma and the potential value of PIK3CA/siRNA chitosan nanoparticle in suppressing the metastasis of gastric carcinoma.
METHODSGastric cancer cells were treated with PIK3CA/siRNA nanoparticle (with a diameter of 350 nm), and the efficiency of PIK3CA gene interference was evaluated using Western blotting and real-time PCR. The changes of the invasive capacity of the treated cells was assessed with Transwell assay.
RESULTSPIK3CA/siRNA chitosan nanoparticle efficiently lowered the expression level of PIK3CA and significantly decreased the invasion of BGC823 cells.
CONCLUSIONPIK3CA gene interference mediated by PIK3CA/siRNA chitosan nanoparticle can decrease the invasive capacity of gastric cancer cells in vitro.
