Effect of CD133(+) cells on the radiosensitivity of rectal cancer cells.
- Author:
Jianming QIU
1
;
Guangen YANG
2
,
3
;
Zhong SHEN
1
;
Lei DING
1
;
Dong WANG
1
;
Chen WANG
1
Author Information
- Publication Type:Journal Article
- MeSH: AC133 Antigen; Adenocarcinoma; metabolism; pathology; radiotherapy; Aged; Animals; Antigens, CD; metabolism; Apoptosis; radiation effects; Cell Line, Tumor; Glycoproteins; metabolism; Humans; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Middle Aged; Neoplasm Transplantation; Peptides; metabolism; Radiation Tolerance; Random Allocation; Rectal Neoplasms; metabolism; pathology; radiotherapy; Tumor Burden; radiation effects
- From: Chinese Journal of Oncology 2014;36(2):103-108
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of CD133(+) cells on radiosensitivity of rectal cancer cells.
METHODSIn vitro experiments: CD133(+) cells were purified with Immunomagnetic beads from human rectal cancer cell line SW480 cells and annexin V/PI staining was used to determine apoptosis in CD133(+) and CD133(-) cells. In vivo experiments: Transplanted rectal tumor was established in 30 nude mice using primarily established SW480 cells. The tumor cells were divided into CD133-high and CD133-low groups based on the immunohistochemical staining of CD133 expression of the tumor cells. The tumor size after irradiation was recorded every three days.
RESULTSCD133(+) cells had a much lower percentage of apoptosis after radiation exposure compared with CD133(-) cells [(12.6 ± 3.2) % vs. (38.8 ± 6.7) %, P < 0.01]. In vivo experiment showed that the normalized tumor size of CD133-high group (3.00 ± 0.32) became significantly larger than that of the CD133-low group(2.55 ± 0.29) at the ninth day and this difference lasted until the observation end (P < 0.05).
CONCLUSIONSCD133(+) cells have a radioresistant effect on rectal cancer cells and may become a potential therapeutic target in the radiotherapy of rectal cancer.
