Absence of evidence for the association of single nucleotide polymorphisms in Annexin A1 gene with type 2 diabetes in Chinese.
- Author:
Yan DONG
1
;
Guo LI
;
Tian-hong LUO
;
Gang WU
;
Wei HUANG
;
Min LUO
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Annexin A1; genetics; Asian Continental Ancestry Group; genetics; China; Diabetes Mellitus, Type 2; ethnology; genetics; Exons; genetics; Female; Genotype; Humans; Introns; genetics; Male; Middle Aged; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Promoter Regions, Genetic; genetics
- From: Chinese Journal of Medical Genetics 2004;21(5):508-511
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify single nucleotide polymorphisms (SNPs) in the annexin A1(ANXA1) gene and to analyze the association of these SNPs with type 2 diabetes in Shanghai Han population.
METHODSSNPs in the promotor and exon regions (including intron sequence near splicing site) in the ANXA1 gene were screened by direct sequencing in 24 type 2 diabetes patients and were further genotyped by direct sequencing in another 171 type 2 diabetes patients and 189 normal control subjects.
RESULTSThe total sequence of ANXA1 gene is 6798 bp. And 7 SNPs were found; among them, 2 SNPs (-7974 C>T and -7040 G>T) were in promotor region, 3 SNPs in intron regions (+9059 A>G, +9204 C>T, +10486 A>G), 1 SNP in 5'-untranslation region (-6614 A>G) and 1 SNP in coding regions (+1784 A>G). These 7 SNPs were genotyped further and the results revealed that the allele frequencies of these SNPs showed no significant difference between the diabetic and the control groups (P>0.05).
CONCLUSIONThere is no association of these SNPs in ANXA1 gene with type 2 diabetes in Shanghai Han population.
