Immune regulation effect of rat dendritic cells phagocytosing photochemotherapy-treated allogeneic cells on syngeneic T cells.
- Author:
De-Hua ZHENG
1
;
Yu-Xiang WEI
;
Bing-Yi SHI
;
Yi-Ping ZOU
;
Guo-Sheng DU
;
Zhi-Dong ZHU
;
Ji-Yong SONG
;
Ying-Chang SHI
;
Li LI
Author Information
1. Chinese PLA Transplant Center, The Second Affiliated Hospital, Chinese PLA General Hospital, Beijing 100091, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Dendritic Cells;
cytology;
immunology;
physiology;
Flow Cytometry;
Isoantigens;
Phagocytosis;
immunology;
Photochemistry;
Rats;
Rats, Inbred Lew;
T-Lymphocytes;
immunology
- From:
Journal of Experimental Hematology
2009;17(6):1492-1496
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study was to investigate the immune regulatory effect of dendritic cells phagocytosing photochemotherapy-treated allogeneic spleen lymphocytes on syngeneic T cells. DA rat spleen lymphocytes were treated with 8-methoxypsoralen plus UVA irradiation (PUVA). LEW rat bone marrow-derived DCs were co-cultured with PUVA-treated DA spleen lymphocytes (PUVA-SP), and the surface markers (MHC-II, CD86 and CD40) of treated DC were detected by flow cytometry. CFSE-labeled PUVA SP were incubated with LEW DCs and the phagocytosis of DCs on PUVA-SP was observed by using fluorescent microscope. The ability of DC phagocytosing allogeneic PUVA-SP (PUVA-SP DC) to stimulate the proliferation of LEW T cells was analyzed by mixed leukocyte reactions (MLR). The production of IL-4, IL-10, IL-2, IFN-gamma in MLR culture supernatant was determined by luminex method. The results indicated that the PUVA treatment effectively induced early apoptosis of DA rat spleen lymphocytes. After co-culture, DC efficiently phagocytosed allogeneic PUVA-SP and still maintained an immature phenotype with low levels of MHC II, CD40 and CD86. PUVA-SP DC induced LEW T cell hyporesponsiveness to DA rat antigen, and led to skewing of T cell cytokine expression toward Th2 (IL-10 and IL-4). It is concluded that the PUVA-SP DC effectively down-regulate T cell response to alloantigen and induce Th2 immune deviation in vitro.
