Dahuang zhechong pill containing serum inhibited platelet-derived growth factor-stimulated vascular smooth muscle cells proliferation by inducing G1 arrest partly via suppressing protein kinase C α-extracellular regulated kinase 1/2 signaling pathway.
- Author:
Na LIU
1
;
Jun-tian LIU
;
Yuan-yuan JI
;
Pei-pei LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta, Thoracic; cytology; Blood Proteins; pharmacology; Cell Proliferation; drug effects; Cells, Cultured; Cyclin D1; metabolism; Cyclin-Dependent Kinase Inhibitor p27; metabolism; DNA; biosynthesis; Drugs, Chinese Herbal; pharmacology; G1 Phase; drug effects; physiology; MAP Kinase Signaling System; physiology; Male; Muscle, Smooth, Vascular; cytology; drug effects; enzymology; Platelet-Derived Growth Factor; pharmacology; Protein Kinase C-alpha; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese journal of integrative medicine 2012;18(5):371-377
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate effects of dahuang zhechong pill ( DHZCP) on the cell cycle and the related signal pathways in vascular smooth muscle cells (VSMCs) stimulated by platelet-derived growth factor (PDGF) with the method of serum pharmacology.
METHODSDNA synthesis in VSMCs was examined by detecting 5'-bromo-2'-deoxyuridine incorporation with the immunocytochemical method. The cycle of VSMCs was evaluated with flow cytometry. Expressions of cyclin D1, p27, protein kinase Cα (PKCα), and phosphorylated extracellular signal regulated kinase 1/2 (ERK1/2) were quantified by Western blot method.
RESULTSDHZCP containing serum significantly inhibited DNA synthesis of PDGF-stimulated VSMCs, arrested the cells in G G(1) phase, modulated the protein expressions of cyclin D D(1) and p27, and suppressed the activation of PKCα and ERK1/2.
CONCLUSIONDHZCP containing serum inhibits VSMCs proliferation via modulating the expressions of cell cycle proteins to arrest the cell in G G(1) phase, which is attributed to, at least in part, suppressing PKCα-ERK1/2 signaling in VSMCs.
