Research Progress of the Differentiation of 3T3-L1 Preadipocytes into Mature Adipocytes.
10.3881/j.issn.1000-503X.2017.05.021
- VernacularTitle:3T3-L1脂肪细胞诱导分化的研究进展
- Author:
Xiu LIU
1
;
Xi-Nong LIU
1
;
Tian-Jia LI
1
;
Bao LIU
1
Author Information
1. Department of Vascular Surgery,PUMC Hospital,CAMS and PUMC,Beijing 100730,China.
- Publication Type:Journal Article
- From:
Acta Academiae Medicinae Sinicae
2017;39(5):727-731
- CountryChina
- Language:English
-
Abstract:
Along with the increase in the prevalence of lipid metabolism disorders,in vitro research on the adipocytes has been a hot topic in recent years. 3T3-L1 preadipocyte line is one of the most commonly used cell line for establishing adipocytes models. However,for 3T3-L1 cell lines,the traditional method,known also as the "Cocktail" method,have disadvantages including low transformation rate and heterogeneous conversion. Therefore,many studies aimed to improving the induction method by changing the time of inducers or adding other new drugs have been performed(known as the improved method). The present study was to summarize the progress of the traditional methods and the improved methods for inducing 3T3-L1 cell lines to mature adipocytes.
