Effects of low shear stress on expression of platelet derived growth factor (PDGF) of organ-cultured artery in vitro.
- Author:
Bo LIU
1
;
Jin ZHOU
;
Zonglai JIANG
;
Yan ZHANG
;
Yanchun LIU
;
Zhiqiang YAN
;
Yuquan LI
Author Information
1. Laboratory of Biomechanics, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Carotid Artery, Common;
chemistry;
metabolism;
Cells, Cultured;
Endothelium, Vascular;
cytology;
metabolism;
Muscle, Smooth, Vascular;
cytology;
metabolism;
Platelet-Derived Growth Factor;
biosynthesis;
Shear Strength;
Stress, Mechanical;
Swine
- From:
Journal of Biomedical Engineering
2005;22(1):5-9
- CountryChina
- Language:Chinese
-
Abstract:
To explore the biological effects of shear stress on intact artery and the change of growth factor during stress-induced vascular remodeling, we established an artery organ-cultured system under stress in vitro, and the common carotid arteries of pigs were cultured under shear stress of 20, 5 and 0 dyn/cm2. PDGF-A synthesis of vascular smooth muscle cells (VSMCs) cultured for 1, 4 and 7 days were studied by immunohistochemical and computer image processing methods, and PDGF-B secretion of endothelial cells (ECs) cultured within 12 h were studied by ELISA. Results showed that PDGF-B increased obviously under shear stress of 5 dyn/cm2, and reached the highest point at about 3 h; PDGF-A synthesis also obviously increased under low shear stress in 7 days. Increasing of PDGF synthesis promotes phenotype switch and proliferation of VSMC. It may have important influence on artery remodeling under low shear stress.
