Beneficial effects of liver X receptor agonist on adipose-derived mesenchymal stem cells transplantation in mice with myocardial infarction
10.3760/cma.j.issn.0253-3758.2012.09.002
- VernacularTitle:肝X受体激动剂可改善间充质干细胞移植治疗小鼠急性心肌梗死的疗效
- Author:
Chun-Hong LI
1
;
Hong-Li DUAN
;
Wei-Wei FAN
;
Ya-Bin WANG
;
Zheng ZHANG
;
Rong-Qing ZHANG
;
Qing-Ting BU
;
Xiu-Juan LI
;
Feng CAO
Author Information
1. 解放军第五四六医院
- Keywords:
Myocardial infarction;
Mesenchymal stem cell transplantation;
Liver X receptor agonists
- From:
Chinese Journal of Cardiology
2012;40(9):723-728
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of liver X receptor (LXR) agonist on adiposederived mesenchymal stem cells (AD-MSCs) implantation into infarcted hearts of mice.Method AD-MSCFluc+ which stably expressed firefly luciferase (Fluc) were isolated from β-actin-Fluc transgenic mice and characterized by flowcytometry.Male FVB mice were randomly allocated into the following four groups (n=10each):(1) sham group; (2) MI + PBSgroup; (3) MI + AD-MSCFluc+ group; (4) MI + AD-MSCFluc + LXR agonist (T0901317) group.AD-MSCFluc+ or PBS were injected intramyocardially into periinfarcted region of mice heart after permanent left anterior descending ( LAD ) artery ligation.Bioluminensence imaging (BLI)was performed for quantification of injected cells retention and survival.Cardiac function was evaluated by echocardiography.Results The AD-MSCFluc+ were positive for CD44 and CD90 by flowcytometry.BLI evidenced the firefly luciferase expression of AD-MSCFluc+ which was positively correlated with cell numbers ( r2 =0.98 ).The results of BLI in vivo revealed that LXR agonist could improve the survival of AD-MSCFluc+ at day 7,14 and 21 after transplantation compared with AD-MSCFluc+ alone group.Cardiac function was further improved in combination therapy group compared with AD-MSCFluc+ alone group (P＜0.05).Conclusions LXR agonist T0901317 can improve the retention and survival of intramyocardial injected AD-MSCFluc+ post-MI,and the combination therapy of T0901317 and AD-MSCFluc+ has a synergetic effect on improving cardiae funetion in this model.
