Effects of genistein on expression of TGF-beta1 and intracellular free Ca2+ concentration in fibroblasts derived from human hypertrophic scar.
- Author:
Chuan CAO
1
;
Shi-Rong LI
;
Xia DAI
;
Zhi FENG
;
Shan XIA
;
Yan-Qing CHEN
;
Heng YAO
Author Information
- Publication Type:Journal Article
- MeSH: Calcium; analysis; Cells, Cultured; Cicatrix, Hypertrophic; metabolism; Fibroblasts; drug effects; metabolism; Genistein; pharmacology; Humans; Transforming Growth Factor beta1; metabolism
- From: Chinese Journal of Plastic Surgery 2008;24(5):335-338
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of Genistein on TGF-beta1 expression and the intracellular free Ca2+ concentration in human hypertrophic scar fibroblasts, and to discuss the mechanism of the anti-fibrosis effect.
METHODSFibroblasts were derived from human hypertrophic scar tissue and cultured in vitro. Genistein in different concentrations (25, 50, 100 micromol/L) was administrated to the fibroblasts, respectively. After 48 hours of co-culture, the expression of TGF-beta1 mRNA and protein were examined by RT-PCR and Western-Blot assay respectively. The intracellular free Ca2+ concentration in hypertrophic scar fibroblasts pretreated by Genistein was determined by laser confocal scanning microscopy with or without the stimulation of bFGF.
RESULTSGenistein inhibited the expression of TGF-beta1 in hypertrophic scar fibroblasts on a concentration-dependent manner. bFGF significantly elevated the intracellular free Ca2+ concentration, however its stimulating effect was remarkably alleviated when the fibroblasts were pre-treated by Genistein.
CONCLUSIONSGenistein can reduce the expression of TGF-beta1 and block the accumulation of intracellular free calcium induced by growth factors. It maybe one of the possible mechanisms of Genistein's antifibrosis effect.
