Study on vitrification of porcine embryos by open pulled straw method.
- Author:
De-Fu ZHANG
1
;
Dong LIU
;
Hua-Li WU
;
Xiao-Feng ZHENG
;
Zhao-Kai WANG
;
Shao-Bing WANG
Author Information
1. Animal Husbandry and Veterinary Research Institute, Shanghai Academy of Agricultural Sciences, China. zhangdefu10@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Blastocyst;
physiology;
Cryopreservation;
methods;
veterinary;
Embryo Transfer;
Female;
Pregnancy;
Swine;
embryology
- From:
Chinese Journal of Biotechnology
2006;22(5):845-849
- CountryChina
- Language:Chinese
-
Abstract:
291 embryos (Blastocyst/Morula) from 20 donor sows were vitrified by two step method with OPS (open pulled straw) in solution I (TCM199 + 20% FBS + 10% EG + 10% DMSO) for 3min, and solution II (TCM199 + 20% FBS + 20% EG + 20% DMSO + 0.4mol/L SUC) for 1min, stored in liquid nitrogen for 3 months, and transferred into 8 recipient sows after warming, one recipient sow was pregnant and 8 alive piglets were born. This is the first paper to report getting alive piglets by vitrification in China.
