Screening of proteins binding to FXR1P using yeast two-hybrid technique.
- Author:
Jiao SU
1
,
2
;
Shu-ya HE
;
Bin-yuan LI
;
Yun MA
;
Chang-shun YU
Author Information
- Publication Type:Journal Article
- MeSH: Autoantigens; genetics; metabolism; Estradiol Dehydrogenases; genetics; metabolism; Ferritins; genetics; metabolism; Gene Library; Humans; Membrane Proteins; genetics; metabolism; Protein Binding; Protein Interaction Domains and Motifs; genetics; RNA-Binding Proteins; genetics; metabolism; Two-Hybrid System Techniques
- From: Journal of Southern Medical University 2009;29(12):2394-2400
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen the proteins interacting with FXR1P for functional investigation of FXR1P.
METHODSThe yeast strain AH109 transformed with the recombinant expression vector pGBKT7/FXR1 was mated with the yeast strain Y187 pretransformed with human fetal brain cDNA library. The positive clones were screened and identified by sequence analysis.
RESULTSThe recombinant expression vector pGBKT7/FXR1 was constructed successfully. Five proteins binding to FXR1P were screened from human fetal brain cDNA library using the yeast two-hybrid system, including CMAS, FTH1, GOLGA4, HSD17B1 and CSH1.
CONCLUSIONSThese results provide new clues for investigating the biological functions of FXR1P and the pathogenesis of Fragile X syndrome.
