Establishment of mouse model of humoral immune response using rabbit red blood cells as the antigen.
- Author:
Cheng-feng QIU
1
;
Lin-sheng LEI
;
Yu-ying WU
;
Chuan-lin YU
;
Zheng-guang ZHU
;
Na-na CHEN
;
Shu-guang WU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Erythrocytes; immunology; Female; Guinea Pigs; Hemolysin Proteins; blood; Immunity, Humoral; Immunization; Male; Mice; immunology; Models, Animal; Rabbits
- From: Journal of Southern Medical University 2009;29(12):2473-2476
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a mouse model of humoral immune response by immunization with rabbit red blood cells (RRBCs).
METHODSThe mice were immunized with RRBCs and the serum hemolysin level was measured by micro-hemolysis spectrophotometry.
RESULTSThe peak time needed for hemolysin production against RRBCs was 6 days after the immunization, and 20% RRBCs in a total volume of 0.2 ml was optimal for intraperitoneal injection. Hydrocortisone (25 mg/kg) and cyclophosphamide (20 mg/kg) inhibited hemolysin production. Mannatide (4 mg/kg) produced no significant effect on serum hemolysin level in normal mice, but significantly potentiated hemolysin production in immunosuppressed mice induced by cyclophosphamide (20 mg/kg).
CONCLUSIONIntraperitoneal RRBC injection is feasible for establishing mouse models of humoral immune response.
