Expression and antiviral assay of bovine interferon-gamma.
- Author:
Zhengzhong 'U
1
;
Xiang CHEN
;
Fengli SHAN
;
Chuang MENG
;
Lin SUN
;
Jinlin HUANG
;
Zhiming PAN
;
Shizhong GENG
;
Xinan JIAO
Author Information
1. Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou 225009, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antiviral Agents;
pharmacology;
Baculoviridae;
genetics;
metabolism;
COS Cells;
Cattle;
Cercopithecus aethiops;
Escherichia coli;
genetics;
metabolism;
Genetic Vectors;
genetics;
Interferon-gamma;
biosynthesis;
genetics;
pharmacology;
Recombinant Proteins;
biosynthesis;
genetics;
pharmacology;
Transfection
- From:
Chinese Journal of Biotechnology
2011;27(2):269-276
- CountryChina
- Language:Chinese
-
Abstract:
Bovine interferon-gamma (BoIFN-gamma) gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) from total RNA of bovine spleen lymphocytes stimulated with ConA. The products of RT-PCR were cloned into pVAX1 vector, positive recombinant clone was identified by restriction enzyme digestion and sequencing. The recombinant plasmid pVAX1-BolFN-gamma was transfected into COS-7 cells mediated by lipofectine, indirect immunofluorescent assay analysis confirmed that rBoIFN-gamma was expressed in COS-7 cells. BoIFN-gamma gene (without signal peptide) was cloned into pET-30a(+) and pGEX-6p-1 vector, and transformed into the Escherichia coli cells. After optimizing the induction condition, SDS-PAGE analysis showed that the expression products were all found in soluble form and had a molecular weight of 23 kDa and 43 kDa respectively. BoLFN-gamma precursor gene (with signal peptide) was cloned into transfer vector pFastBac 1, and transformated into DH10Bac E. coli cells. By site-specific transposition, BoIFN-gamma gene was integrated into shuttle vector Bacmid, and transfected into the Sf9 insect cells mediated by lipofectine to produce recombinant baculovirus. Indirect immunofluorescent assay analysis confirmed that rBac-BoLFN-gamma was expressed successfully in Baculovirus vector system. The antiviral activities of rHis-BoIFN-gamma, rGST-BoIFN-gamma and rBac-BoIFN-gamma were up to 8.389 x10(7) U/mg, 6.554 x10(5) U/mg and 4.096 x 10(4) U/mL respectively, which were analyzed in MDBK/VSV system. A sandwich ELISA was established using monoclonal antibodies 3E6 and 5G4, which can detect BoIFN-gamma in quantity and provide a useful method for the clinical practice and research of BolFN-gamma.
