Transfection with human adenovirus type 5 early-region 1A protects the primary human fibroblasts from ras-induced premature senescence in vitro.
- Author:
Yi-lei LI
1
;
Ping ZHENG
;
Chong-yuan XU
;
Yuan YAN
Author Information
- Publication Type:Journal Article
- MeSH: Adenovirus E1A Proteins; genetics; metabolism; Apoptosis; genetics; physiology; Cell Transformation, Neoplastic; genetics; metabolism; Cells, Cultured; Cellular Senescence; genetics; physiology; Fibroblasts; cytology; metabolism; Genes, ras; physiology; Humans; Skin; cytology; Transfection
- From: Journal of Southern Medical University 2008;28(10):1820-1823
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of human adenovirus type 5 (Ad5) early-region 1A (E1A) in premature senescence of human fibroblasts induced by Ras activation.
METHODSHuman fibroblasts were cotransduced with E1A, E1A1-143, or their vector (BP) and Ha-RasV12 or its vector (WH). The growth curves and percentages of the apoptotic cells were determined.
RESULTSExpression of E1A or Ha-RasV12 in human fibroblasts significantly inhibited the cell growth. Transduction of Ha-RasV12 along with E1A or E1A 1-143 into human fibroblast cells resulted in active and rapid cell proliferation.
CONCLUSIONE1A can rescue human fibroblasts from Ras-induced premature senescence, and the senescence bypassing activity of E1A resides in its NH2 terminus.
