Protective effect of epigallocatechin gallate against sperm abnormality in mice.
- Author:
Liu-Cai SUI
1
;
Yi-Feng GE
;
Juan-Juan XU
;
Rong-Hua WU
;
Hai-Yan FU
;
Bing YAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Catechin; analogs & derivatives; pharmacology; Cyclophosphamide; toxicity; Male; Mice; Mutagens; toxicity; Random Allocation; Spermatozoa; drug effects; Time Factors
- From: National Journal of Andrology 2014;20(12):1068-1072
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effect of epigallocatechin gallate (EGCG) on mouse sperm in vivo.
METHODSA total of 64 six-week-old male Kuming mice were randomly divided into eight groups of equal number to be treated with normal saline (negative control), Cyclophosphamide (CP) at 30 mg/kg (positive control), and CP followed by EGCG (experimental) at 20, 40, and 80 mg/kg, respectively, given every other day for 10 days. At 4 and 5 weeks after treatment, the bilateral testes of the mice were harvested for examination of sperm abnormality.
RESULTSEGCG did not increase the rate of CP-induced sperm abnormality in the mice, but reduced it instead with the prolonged time of treatment.
CONCLUSIONEGCG protects mouse sperm in vivo.
