Screening of plasma tumor biomarkers for hypopharyngeal carcinoma by proteomics method.
- Author:
Junzheng LI
1
;
Wendong TIAN
;
Xiong LIU
;
Shuiwang HU
;
Bao ZHANG
;
Xiangping LI
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; blood; Blood Proteins; metabolism; Humans; Hypopharyngeal Neoplasms; blood; Male; Middle Aged; Proteomics; methods
- From: Journal of Southern Medical University 2013;33(8):1173-1176
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen tumor biomarkers in the plasma close related with hypopharyngeal carcinoma.
METHODSPooled plasma from 6 patients with hypopharyngeal carcinoma and 6 healthy individuals were collected. After removal of high-abundance plasma proteins, two-dimensional gel electrophoresis (2-DE) was performed to isolate the total proteins, and the protein spots with more than 2-fold differential expressions were detected by 2D analysis software followed by identification by MALDI-TOF/TOF mass spectrometer. Western blotting was performed to validate the expression level of α2-HS-glycoprotein.
RESULTSA total of 11 differentially expressed protein spots were selected, including 5 upregulated proteins and 6 downregulated proteins. MALDI-TOF/TOF identified the upregulated proteins in hypopharyngeal carcinoma patients as alpha-2-HS-glycoprotein and haptoglobin and downregulated ones as Ig kappa chain C region and apolipoprotein A-I. Western blotting demonstrated that α-2-HS- glycoprotein expression level was consistent with that detected by 2-DE.
CONCLUSIONPatients with hypopharyngeal carcinoma show different plasma protein profiles from healthy individuals. These differentially expressed proteins may serve as potential specific tumor biomarkers for hypopharyngeal carcinoma.
