Histone deacetylase inhibitors induce human renal cell carcinoma cell apoptosis through p-JNK activation.
- Author:
Miqing XU
1
,
2
;
Ming HONG
;
Hui XIE
Author Information
- Publication Type:Journal Article
- MeSH: Anthracenes; pharmacology; Antineoplastic Agents; administration & dosage; pharmacology; Apoptosis; drug effects; Carcinoma, Renal Cell; metabolism; pathology; Cell Cycle; drug effects; Cell Line, Tumor; Cell Survival; Dose-Response Relationship, Drug; Histone Deacetylase Inhibitors; administration & dosage; pharmacology; Humans; Hydroxamic Acids; administration & dosage; pharmacology; Indoles; administration & dosage; pharmacology; JNK Mitogen-Activated Protein Kinases; metabolism; Kidney Neoplasms; metabolism; pathology; MAP Kinase Signaling System; drug effects; Phosphorylation; Proto-Oncogene Proteins c-bcl-2; metabolism; bcl-2-Associated X Protein; metabolism
- From: Journal of Southern Medical University 2013;33(10):1409-1415
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the effect of histone deacetylase inhibitors trichostatin A (TSA) and LBH589 on the growth of human renal cell carcinoma OS-RC-2 cells in vitro and explore the underlying molecular mechanism.
METHODSOS-RC-2 cells were treated with LBH589 or TSA with or without SP600125 pretreatment, and the cell viability was measured by MTT assay. The changes of cell cycle distribution and apoptosis of OS-RC-2 cells were examined by flow cytometry, and the expressions of c-Jun, p-c-Jun, Bcl-2, and Bax were quantified by Western blotting.
RESULTSTSA and LBH589 both inhibited the growth of OS-RC-2 cells in a dose- and time-dependent manner. TSA at 1 µnmol/L and LBH589 at 50 nmol/L caused obvious cell cycle arrest in G2/M phase and cell apoptosis, and significantly increased the protein levels of phosphorylated c-Jun. TSA treatment obviously increased Bax expression but decreased Bcl2 expression in the cells. The growth inhibitory effect of TSA was attenuated by the JNK inhibitor SP600125 in OS-RC-2 cells. TSA-induced phosphorylation of c-Jun and Bax upregulation was partially counteracted by SP600125.
CONCLUSIONTSA and LBH589 can cause cell cycle arrest and induce apoptosis in OS-RC-2 cells, in which process P-JNK pathway plays an important role.
