A Novel SELEX method for screening of HIV-1 P24 antigen

VernacularTitle:筛选HIV-1 P24蛋白适配子的一种简便的SELEX方法
Author: Shao-Bing ZHAN ¹ ; Xiao-Guang ZHANG ; Hong-Xia LI ; Yi ZENG
Author Information

1. 100052 北京,中国疾病预防控制中心病毒病预防控制所肿瘤室,传染病预防控制国家重点实验室
Keywords: HIV; SELEX; Aptamer
From: Chinese Journal of Experimental and Clinical Virology 2013;27(3):218-220
CountryChina
Language:Chinese
Abstract: Objective To screen aptamers that can bind P24 antigen tightly and specificly,and verify its specificity and affinity.Methods Polycarbonate PCR plate was coated with P24 antigen and SELEX technology was used to screen aptamer on the PCR plate.The primary and secondary structure of these aptamers was analyzed by software.Through HRP labeled streptavidin and biotin labeled aptamers,the affinity and specificity of obtained aptamers were verified by ELISA.Results The polycarbonate PCR plate could be coated with P24 antigen.Electrophoretic analysis showed the aptamers had been enriched.Sequence aligment analysis showed that these aptamers have consensus sequence and their apatial structure was multiple; ELISA verified that aptamers had high affinity with P24 antigen.Conclusion A simple method was established for screening aptamers that can bind HIV-1 P24 antigen specificly and tightly.