Increased Expression of PI-3K in Asthmatic Rat T Lymphocytes
- Author:
Jin LIU
1
;
Shixin ZHOU
;
Shengdao XIONG
;
Yongjian XU
;
Zhenxiang ZHANG
;
Weining XIONG
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
PI-3K;
asthma;
T helper 2 cell;
interleukin-4
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(1):34-36
- CountryChina
- Language:Chinese
-
Abstract:
In order to explore the expression of PI-3K in T lymphocytes of asthmatic rats and the relationship between PI-3K and activation of T lymphocytes, 24 Wistar rats were randomly divided into 4 groups: normal control group, asthmatic one-week group, asthmatic two-week group and asthmatic four-week group. T cells were purified from blood of each rat and the expression of PI-3K was observed by immunocytochemical fluorescence staining, the semiquantitative fluorescence intensity was measured by HPIAS-2000 analytic software, and the expression of IL-4 in supernatants was detected by ELISA. The results showed that the fluorescence intensity of T lymphocytes in asthmatic groups was significantly higher than that in normal control (P＜0.001), indicating that the expression of PI-3K in T lymphocytes of asthmatic rats was significantly higher than that in those of normal controls, and the difference between acute and chronic stage asthmatic groups was significant (P＜0.05). The expression levels of IL-4 protein in supernatants of asthmatic T lymphocytes were significantly higher than those in the normal controls (P＜0.05). There was a significant positive correlation between the expression of PI-3K in T lymphocytes and the IL-4 protein expression in supernatants (r=0.583, P＜0.01). It was suggested that PI-3K signal pathway may participate in the processes of activation and other cytological effects of asthmatic T lymphocytes, thus may play an important roles in the pathogenesis of asthma.
