Effect of Interleukin-1βon IA and IK Currents in Cultured Murine Trigeminal Ganglion Neurons
- Author:
Jianping PAN
1
;
Lieju LIU
;
Fei YANG
;
Xuehong GAO
;
Hui FU
;
Zhangxin MING
Author Information
1. 华中科技大学同济医学院
- Keywords:
IL-1β;
trigeminal ganglion neurons;
IA current (rapidly activating,rapidly inactivating potassium current);
IK current (delayed rectifier potassium current)
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(2):131-134
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of interleukin-1β (IL-1β) on IA and IK currents in cultured murine trigeminal ganglion (TG) neurons, whole-cell patch clamp technique was used to record the IA and IKcurrents before and after 20 ng/mL IL-1β perfusion. Our results showed that 20 ng/mL IL-1β inhibited IA currents (18.3±10.7)% (n=6, P＜0.05). IL-1β at 20 ng/mL had no effect on G-V curve of IA but moved the H-infinity curve V0.5 from -36.6 ± 6.1 mV to-42.4 ±5.2 m V (n=5, P＜0.01). However, 20 ng/mL IL-1β had effect on neither the amplitude nor the G-V curve of IK. IL-1β was found to selectively inhibit IA current in TG neurons and the effect may contribute to hyperalgesia under various inflammatory conditions.
