Comparision of viremia formation between guinea-pigs infected with wild and attenuated ( SA14-14-2) Japanese encephalitis viruses

VernacularTitle:乙型脑炎野毒株和弱毒株(SA14-14-2)在豚鼠体内的病毒血症比较试验
Author: Xin-Yu LIU ¹ ; Yong-Xin YU ; Hong-Shan XU ; Guo-Dong LIANG ; Huan-Yu WANG ; Li-Li JIA ; Guan-Mu DONG
Author Information

1. 中国药品生物制品检定所
Keywords: Encephalitis,Japanese; Virulence; Models,animal; Toxemia
From: Chinese Journal of Experimental and Clinical Virology 2010;24(5):343-345
CountryChina
Language:Chinese
Abstract: Objective To study the viremia formation in guinea-pigs infected with wild type and attenuated Japanese encephalitis virus (JEV). Methods Guniea pigs were inoculated intraperitoneally with different wild JEV strains and the attenuated vaccine strain and its parent virulent strain. Viremia was detected on different days following virus inoculation. Results All the guinea-pigs inoculated with the wild JEV strains induced different levels of viremia ( 1.00-3.40 lg pfu) on the 1 st and 3rd day post inoculation.Using a virus titer of 104 pfu for inoculation, the animals inoculated with the SA14 parent strain induced relatively high viremia (1024-10 3.4 pfu), however no viremia coulds be detected on any tested days.Conclusion The degree of viremia in guinea pigs can be used as a new method to evaluate the attenuation of JEV.