Proto-oncogene c-src regulates the viability of rat spermatogonial stem cells in vitro through phosphorylated signal transducer and activator of transcription-3.
- Author:
Jia-Xiang CHEN
1
;
Xin-Chang WANG
;
Jing-Lei WANG
;
Si-Fan XU
;
Hai-Yan QIN
;
Bei YANG
;
Jun-Ling YANG
;
Ting ZOU
Author Information
1. Department of Physiology, Medical College of Nanchang University, Nanchang 330006, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
Genes, src;
Male;
Phosphorylation;
Proto-Oncogene Proteins pp60(c-src);
metabolism;
RNA, Messenger;
Rats;
STAT3 Transcription Factor;
metabolism;
Spermatogonia;
cytology;
metabolism;
Stem Cells;
cytology;
metabolism;
Transfection
- From:
Acta Physiologica Sinica
2008;60(3):391-396
- CountryChina
- Language:Chinese
-
Abstract:
The present study aimed to investigate the effect of proto-oncogene c-src on the viability of rat spermatogonial stem cells from 9 day-old rat in vitro. MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense c-src oligodeoxynucleotides (ODNs) in vitro; RT-PCR was utilized to observe the expression of c-src mRNA and Western blot was used to observe the protein expressions of pp60c-src and phosphorylated signal transducer and activator of transcription-3 (p-STAT3). Compared with that in control group, the viability of spermatogonial stem cells decreased by 8.1% (P<0.05) and the expression of c-src mRNA decreased significantly after treatment with 10 μmol/L antisense c-src ODNs for 12 h. Compared with that in the control group, the protein expressions of pp60c-src and p-STAT3 decreased by 33.8% and 45.3% (both P<0.01), respectively, in the spermatogonial stem cells after being transfected with antisense c-src ODNs. The results suggest that proto-oncogene c-src regulates the viability of rat spermatogonial stem cells through p-STAT3.
