Treatment of organ transplant rejection with low doses of CsA and CTLA4-Ig.

Yeben QIAN; Guihua CHEN; Jiefu HUANG

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Treatment of organ transplant rejection with low doses of CsA and CTLA4-Ig.

Author: Yeben QIAN ¹ ; Guihua CHEN ; Jiefu HUANG
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1. Department of General Surgery, First Affiliated Hospital Anhui Medical University, Anhui 230022, China.
Publication Type:Journal Article
MeSH: Abatacept; Animals; Antigens, CD; Antigens, Differentiation; therapeutic use; CTLA-4 Antigen; Cyclosporine; therapeutic use; Disease Models, Animal; Drug Therapy, Combination; Graft Rejection; drug therapy; Immunoconjugates; Immunosuppressive Agents; therapeutic use; Male; Rats; Rats, Sprague-Dawley; Rats, Wistar; Transplantation, Homologous; Transplants
From: Chinese Journal of Surgery 2002;40(2):150-152
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study on treating organ transplant rejection with low doses of CsA and CTLA4-Ig.
METHODSWe set up a rat's cardiac transplant rejection model by Ono's way. The experimental rats were divided into four groups: group A: without any treatment; group B: intraperitoneal injection of CsA at 10 mg / (-1) / (-1) 1 - 7 days; after operation group C: intraperitoneal injection of 100 microgram CTLA4-Ig after 2nd operative day; group D: intraperitoneal injection of CsA 2 mg / (-1) / (-1) 1- 7 days after operation. 50 microgram CTLA4-Ig was given intraperitoneally on the 2nd day postoperation. The survival days of allograft, serum concentration of IL-2, and histological changes were tested.
RESULTSThe allograft survival time of the four groups was 7.2 +/- 0.7 (group A), 19.4 +/- 2.07 (group B), 31.6 +/- 1.8 (group C) and 24.6 +/- 2.07 (group D) respectively. There were significantly differences among the groups (P < 0.05). The survival time in group D was more prolonged than that in group B (P < 0.05). The concentration of IL-2 was significantly decreased after operation. Significant difference was observed between the control group and each treated group (P < 0.01). In group A, B, C and D allograft rejection was graded IV, II, I and I respectively.
CONCLUSIONCTLA4-Ig had a stronger immunosuppression than did CsA. The low doses of CsA and CTLA4-Ig had show a synergistic immunosuppression in allograft transplantation.