Expression of hypoxia inducible factor-1α and correlated target genes in human laryngeal carcinoma
10.3760/cma.j.issn.1673-0860.2012.04.016
- VernacularTitle:组织缺氧诱导因子-1α及相关靶基因在喉鳞状细胞癌组织中的表达
- Author:
Ou XU
1
;
Xiao-Ming LI
;
Mao-Xin WANG
;
Yong-Tao QU
;
Xiu-Ying LU
;
Chun-Guang SHAN
;
Qing-Jia SUN
Author Information
1. 河北医科大学
- Keywords:
Laryngeal neoplasms;
Carcinoma,squamous cell;
Hypoxia-inducible factor 1,alpha subunit;
Glucose transporter type Ⅰ
- From:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
2012;47(4):330-333
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of hypoxia inducible factor 1 alpha (HIF-1α),glucose transporter protein-1 (GLUT-1) and vascular endothelial growth factor (VEGF) in human laryngeal carcinoma tissue,and to study the relationship between hypoxia and HIF-1α,GLUT-1,VEGF in human laryngeal carcinoma Hep-2 cells and to explore the effect of HIF-1α,GLUT-1 and VEGF as endogenous hypoxic markers on laryngeal carcinoma.Methods The expression levels of HIF-1α,GLUT-1 and VEGF were detected in 35 cases of laryngeal carcinoma by SP immunohistochemical methods and in Hep-2 cells by SP immunocytochemical methods.The relationship between HIF-1 α and GLUT-1,VEGF protein expression was analyzed.Results Of the 35 cases,16 cases expressed HIF-1α,16 cases expressed GLUT-1,19 cases expressed VEGF.The expression of HIF-1α and VEGF were closely correlated with pathologic grading and lymphnode metastasis.GLUT-1 was correlated with lymphnode metastasis.The expression levels of HIF-1 α,GLUT-1 and VEGF in Hep-2 cells under hypoxic condition were higher than those under normoxiccondition.Conclusion HIF-1α may promote the expression of GLUT-1 and VEGF in laryngeal carcinoma,furthermore promote tumor angiogenesis,invasion,and metastasis of the laryngeal carcinoma.
