Preparation of a biphasic calcined bone of beta-TCP/HAP and its application in BMP-2 gene medicine.
- Author:
Xiaoliang XU
1
;
Tingting TANG
;
Jueren LOU
;
Chaofeng YU
;
Kerong DAI
Author Information
1. Department of Orthopaedics, Ninth People's Hospital, Shanghai Second Medical University, Shanghai
- Publication Type:Journal Article
- MeSH:
Animals;
Biodegradation, Environmental;
Bone Morphogenetic Protein 2;
Bone Morphogenetic Proteins;
genetics;
Bone Regeneration;
drug effects;
Bone Substitutes;
chemistry;
pharmacology;
Calcium Phosphates;
pharmacology;
Cell Differentiation;
Goats;
Hydroxyapatites;
pharmacology;
Materials Testing;
Mesenchymal Stromal Cells;
cytology;
Mice;
Mice, Nude;
Transfection;
Transforming Growth Factor beta
- From:
Journal of Biomedical Engineering
2003;20(4):630-633
- CountryChina
- Language:Chinese
-
Abstract:
In this study, we produced a biphasic absorbable calcined bone (CB) of beta-TCP/HAP, and evaluated its function as a carrier of bone marrow derived mesenchymal stem cells(BMSCs) in BMP-2 gene medicine. Biphasic CB was manufactured and its surface was coated by collagen. X-ray diffraction analysis, scanning electron microscopy and biomechanical measurement were performed on the product. Heterotopic bone induction of product as carrier of BMP-2 gene transferred BMSCs and its biodegradability were tested in nude mice and goats. X-ray diffraction analysis showed biphasic patterns of HAP and beta-TCP. Scanning electron microscopy showed the porosity were similar to those of the cancellous bone, and the adhesion of cells on the CB surface were better after surface-coating with collagen. It had certain biomechanical strength and appropriate biodegradability. Biphasic CB loaded with Adv-hBMP-2 transduced BMSCs could induce much bony callus at the subcutaneous site of nude mice and the tibial bone defects of goats. The results showed biphasic CB is a superior carrier of cells in BMP-2 gene medicine.
