Effect of Homoharringtonine Combined with Imatinib on the K562/G01 Cells and Its Mechanism.
- VernacularTitle:高三尖杉酯碱联合伊马替尼对K562/G01细胞的作用及机制研究
- Author:
Jing-Jing WU
1
;
Yi-Han DING
1
;
Zhi-Kui DENG
1
;
Yu-Ye SHI
1
;
Xue-Ying LU
1
;
Yu-Feng LI
2
Author Information
- Publication Type:Journal Article
- From: Journal of Experimental Hematology 2017;25(1):80-84
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of homoharringtonine(HHT) combined with imatinib(IM) on proliferation and apoptosis of K562/G01 cells and its potential mechanism.
METHODSK562/G01 cells were cultured with HHT and/or IM. CCK-8 assay was used to detect cell proliferation. Cell apoptosis and phosphorylated tyrosine levels were analyzed by flow cytometry. The expression levels of p210, PI3K, p-Akt and Akt protein were determined by Western blot.
RESULTSCompared with HHT or IM alone, drug combination significantly inhibited cell proliferation and induced apoptosis of K562/G01 cells (both P< 0.05). HHT combined with IM could inhibit the levels of phosphorylated tyrosine and phosphorylated Crkl and downregulate the expressions of p210, PI3K and p-Akt in K562/G01 cells.
CONCLUSIONHHT combined with IM can synergistically inhibit proliferation and induce apoptosis of K562/G01 cells by suppressing the p210 expression and its kinase activity.
