Effects of Biejia Ruangan Tablet-containing serum on matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 expression in cultured renal interstitial fibroblasts.
- Author:
Jin ZHOU
1
,
2
;
Xiang-mei CHEN
;
Shu-wen LIU
;
Bo FU
;
Quan HONG
;
Shu-juan WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Collagen Type I; genetics; metabolism; Collagen Type III; genetics; metabolism; Drugs, Chinese Herbal; pharmacology; Fibroblasts; drug effects; metabolism; Gene Expression Regulation; drug effects; Humans; Kidney; cytology; Male; Matrix Metalloproteinase 9; genetics; metabolism; RNA, Messenger; genetics; metabolism; Rats, Sprague-Dawley; Serum; metabolism; Tablets; Tissue Inhibitor of Metalloproteinase-1; genetics; metabolism; Transforming Growth Factor beta1; pharmacology
- From: Chinese journal of integrative medicine 2015;21(2):152-156
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of Biejia Ruangan Tablet ([symbol in text], BRT)-containing serum on the expression of matrix metalloproteinase (MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) in cultured renal interstitial fibroblasts.
METHODSDifferent BRT-containing sera were prepared by gastric gavages to rats with the high-dose (7 g/kg), mid-dose (3.5 g/kg), and low-dose (1.75 g/kg) BRT respectively. The expression of extracellular matrix in NRK-49F cells was induced by treatment with human transforming growth factor-β1 (recombined human TGF-β1), and BRT-containing serum. Western blotting and Northern blotting were used to measure type I and III procollagen, MMP-9, and TIMP-1.
RESULTSThe high dose BRT-containing serum could decrease the type I and III procollagen gene expression which boosted by TGF-β1, at the same time cut down TIMP-1 protein and gene expression which increased by TGF-β1 (P <0.05). Treatment of cells with recombined human TGF-β1 had no significant effect on MMP-9 expression and BRT-containing serum also had no effect on MMP-9 expression.
CONCLUSIONSHigh dose BRT has anti-fibrosis effects in NRK-49F cells, as indicated by its inhibition of type I and III procollagen and TIMP-1 expression.
