Preparation of floxuridine loaded polycation and its antitumor activity.
- Author:
Dan-Jun ZHAO
1
;
Xiao LU
;
Qi-Ying JIANG
;
Dan CHEN
;
Jun ZHOU
;
Hai YU
;
Qing-Qing WANG
;
Gu-Ping TANG
Author Information
- Publication Type:Journal Article
- MeSH: Antimetabolites, Antineoplastic; chemical synthesis; pharmacology; Cell Line, Tumor; Cell Movement; drug effects; Cell Proliferation; drug effects; Floxuridine; pharmacology; Fluorouracil; pharmacology; Humans; Liver Neoplasms; pathology; Polyethyleneimine; pharmacology; Prodrugs; chemical synthesis; pharmacology; beta-Cyclodextrins; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2009;38(1):53-58
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a new prodrug of 5-fluorouracil-polyethylenimine-beta-cyclodextrin-floxuridine (PEI-beta-CyD-Fd) and to test its antitumor activity.
METHODSFloxuridine was conjugated to polyethylenimine-beta-cyclodextrin to form prodrug PEI-beta-CyD-Fd. The structure of synthesized PEI-beta-CyD-Fd was confirmed by (1)H-NMR, FT-IR and UV. MTT assay and cell wound healing assay were performed on human hepatic carcinoma cell line HepG2.
RESULTThe drug loading was 2 %. The MTT assay and cell wound healing assay indicated that PEI-beta-CyD-Fd significantly inhibited proliferation and migration of HepG2 cells.
CONCLUSIONThe synthesized prodrug PEI-CyD-Fd has a significant antitumor activity on HepG2 cells.
