Signal transduction pathway mediated by thrombopoietin in the inflammation model of microglia.
- Author:
Qiao-Jun LI
1
;
Li-Ping ZOU
;
Jian-Wen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Enzyme-Linked Immunosorbent Assay; Extracellular Signal-Regulated MAP Kinases; genetics; metabolism; Inflammation; etiology; Mice; Microglia; pathology; RNA, Messenger; analysis; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; physiology; Thrombopoietin; analysis; genetics; physiology
- From: Chinese Journal of Contemporary Pediatrics 2011;13(4):340-343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the signal transduction pathway mediated by thrombopoietin (TPO) in the inflammation model of microglia induced by lipopolysaccharide (LPS).
METHODSThe inflammation model of microglia BV2 cells was prepared by LPS of 0.5 and 1.0 μg/mL stimulation. The expression of TPO and ERK mRNA in BV2 cells was detected by real time quantitative PCR. Western blot was used to evaluate the expression of TPO and ERK protein in BV2 cells. TPO and IL-6 contents in the culture supernatant fluid were measured using ELISA.
RESULTSLPS stimulation increased significantly the mRNA and protein expression of TPO and ERK in BV2 cells, especially at the concentration of 1.0 μg/mL for 12 hrs stimulation. There was a significant positive correlation between the mRNA and protein expression of TPO and ERK.
CONCLUSIONSSignal transduction pathway of ERK1/2 participates in the activation of TPO in inflammatory injury of BV2 cells.
