Simultaneous determination of four flavonoids in Malus prunifolia from Shanxi province by RP-HPLC.
- Author:
Taigang LIANG
1
;
Enli LIU
;
Chengxiao ZHAO
;
Shurong BAN
;
Qingshan LI
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromatography, High Pressure Liquid; methods; Chromatography, Reverse-Phase; methods; Drugs, Chinese Herbal; analysis; Flavonoids; analysis; Malus; chemistry
- From: China Journal of Chinese Materia Medica 2009;34(17):2217-2219
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for simultaneous determination of rutin, hyperoside, quercetin and kaempferol in Malus prunifolia from Shanxi province in China.
METHODThe separation was performed on a Hypersil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with methanol-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 360 nm and the temperature of column was 25 degrees T.
RESULTThe linear ranges of rutin, hyperoside, quercetin and kaempferol were 1.87-46.67 mg x L(-1), 6.40-160.0 mg x L(-1), 3.33-83.33 mg x L(-1), 0.80-20.00 mg x L(-1), respectively. The average recoveries (n=6) of the four constituents were 99.2% (RSD 2.9%), 98.2% (RSD 1.9%), 97.4% (RSD 2.3%), 97.2% (RSD 1.3%), respectively.
CONCLUSIONThe method was simple, accurate and can be used to evaluate medicinal value of Malus prunifolia.
