Study on chemical constituents of seeds of Croton tiglium and their cytotoxicities.

Author: Hai-Guo SU ¹ ; Huai YANG ¹ ; Chun-Wang MENG ¹ ; Cheng PENG ¹ ; Li GUO ¹ ; Ou DAI ¹
Author Information

1. State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
Publication Type:Journal Article
Keywords: Croton tiglium; chemical constituents; cytotoxic activity; structure identification
From: China Journal of Chinese Materia Medica 2016;41(19):3620-3623
CountryChina
Language:Chinese
Abstract: Seven compounds were isolated from the seeds of Croton tiglium by preparative TLC, semi-preparative HPLC, and column chromatography over silica gel, MCI, and Sephadex LH-20, etc. Their structures were elucidated by spectroscopic data analysis as bis(2,3-dihydroxypropyl) nonanedioate (1), 12-O-(α-methyl)butyrylphorbol-13-decanoate (2), 12-O-tiglylphorbol-13-decanoate (3), (9S,10R,11E,13R)-9,10,13-trihydroxyoctadec-11-enoic acid (4), methyl (9S,10R,11E,13R)-9,10,13-trihydroxyoctadec-11-enoate (5), 4(1H)-quinolinone (6), and 5-hydroxy-2-pyridinemethanol (7). Compound 1 was a new compound and compounds 4-7 were isolated from family Euphorbiaceae for the first time. Compounds 2 and 3 showed cytotoxic activities against human lung cancer cell line A549 with IC₅₀ values of 47.8 and 7.0 μmol•L ⁻¹, respectively, and against human hepatocarcinoma cell line HepG2 with IC₅₀ values of 71.4 and 44.0 μmol•L ⁻¹, respectively.