Effects of IFN alpha on the expressions of Collagen I and transforming growth factor beta 1 in hepatic stellate cell activated by PDGF-BB.
- Author:
Jing HU
1
;
Zhi-wei LI
;
Xiu-jin YANG
;
Yue-jie YANG
;
Li MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Collagen Type I; genetics; metabolism; Dose-Response Relationship, Drug; Gene Expression; Hepatic Stellate Cells; drug effects; metabolism; Interferon-alpha; administration & dosage; pharmacology; Liver Cirrhosis; metabolism; pathology; Platelet-Derived Growth Factor; administration & dosage; pharmacology; Proto-Oncogene Proteins c-sis; RNA, Messenger; genetics; metabolism; Rats; Reverse Transcriptase Polymerase Chain Reaction; methods; Transforming Growth Factor beta1; genetics; metabolism
- From: Chinese Journal of Hepatology 2009;17(9):657-660
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of IFN alpha on the expressions of Collagen I and TGF beta 1 in hepatic stellate cell activated by PDGF-BB.
METHODSHepatic stellate cells (rHSC-99) treated with IFN alpha of different concentration (0, 0.0125, 0.025, 0.050, 0.100, 0.200, 0.400 ng/ml). The cell viability of HSC was measured by MTT. The levels of Col-I mRNA and TGF beta 1 mRNA were measured by the quantitative reverse-transcription polymerase chain reaction (RT-PCR).
RESULTS(1) When HSC was exposed in PDGF-BB, the cell viability of HSC (1.35 +/- 0.22) was higher than that of the control group (0.890 +/- 0.12) (F = 16.311, P less than 0.05), indicating that PDGF-BB can promote the cell viability of HSC. When HSC was exposed to both PDGF-BB and different concentration of IFN alpha (0.025, 0.05, 0.1, 0.2, 0.4 ng/ml), the cell viability of HSC (0.840 +/- 0.18, 0.450 +/- 0.15, 0.260 +/- 0.01, 0.330 +/- 0.07, 0.30 +/- 0.06) were lower than that of the control group (0.890 +/- 0.12) (F = 7.430, P less than 0.05), indicating that the cell viability of HSC was inhibited when HSC was exposed to both PDGF-BB and different concentrations of IFN alpha. Furthermore, within the range of 0.025 ng/ml to 0.1 ng/ml, the effect of IFN alpha was dose-dependent. (2). The relative expression values of Col-I mRNA in different groups of (0.05, 0.1, 0.2 ng/ml) IFN alpha +PDGF-BB are (0.940 +/- 0.19, 0.610 +/- 0.12, 0.520 +/- 0.02), which were lower than those in the control group (1.410 +/- 0.01) (F = 127.921, P less than 0.05). The relative expression values of TGF beta 1 mRNA in different groups of (0.05, 0.1, 0.2 ng/ml) IFN alpha +PDGF-BB are (1.180 +/- 0.06, 1.150 +/- 0.10, 1.390 +/- 0.04), again were lower than those in the control group (1.620 +/- 0.12) (F = 82.115, P less than 0.05). These results indicated that the expression of Col-I mRNA and TGF beta 1 mRNA was remarkably inhibited when HSC was exposed in both PDGF-BB and IFN alpha.
CONCLUSIONThe cell viability of HSC and the expression of Col-I mRNA and TGF beta 1 mRNA is remarkably inhibited when HSC is exposed in both PDGF-BB and IFN alpha, and the inhibition is dose-dependent.
