Calcitonin gene-related peptide induces phenotypic transformation of corpus cavernosum smooth muscle cells in diabetic rats with erectile dysfunction.
- Author:
Shu-hua HE
1
;
An-yang WEI
;
Ting-yu YE
;
Yong YANG
;
Xin-gui LUO
;
Yang LIU
;
Tao ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcitonin Gene-Related Peptide; pharmacology; Calcium-Binding Proteins; metabolism; Cells, Cultured; Diabetes Mellitus, Experimental; complications; genetics; metabolism; Erectile Dysfunction; etiology; genetics; metabolism; Male; Microfilament Proteins; metabolism; Muscle, Smooth; cytology; metabolism; Osteopontin; metabolism; Penis; cytology; drug effects; metabolism; Phenotype; Rats; Rats, Sprague-Dawley
- From: National Journal of Andrology 2011;17(10):913-917
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of the calcitonin gene related peptide (CGRP) on the phenotypic transformation of corpus cavernosum smooth muscle cells (CCSM) in diabetic rats with erectile dysfunction (ED).
METHODSModels of diabetes and diabetic ED were established in male Sprague-Dawley rats by administration of streptozotocin, and CCSMs were primarily cultured and subjected to immunocytochemical assay. The cells were divided into a diabetic ED and a normal control group, and exposed to 0, 10, 60 and 100 nmol/L of CGRP for 24 hours. Then the relative expressions of calponin 1 (Cnn1) and osteopontin (OPN) mRNA were determined by real-time fluorescence quantitative RT-PCR (qRT-PCR).
RESULTSThe rate of SMalpha-actin positive cells in the CCSMs was (95.94 +/- 0.03) %. The expression of Cnn1 mRNA was significantly lower while that of OPN mRNA remarkably higher in the diabetic ED rats (4.41 +/- 0.29 and 5.28 +/- 0.32) than in the normal controls (10.35 +/- 0.62 and 1.32 +/- 0.24) (P < 0.01). Exposure to 100 nmol/L of CGRP significantly upregulated the expression of Cnn1 mRNA and downregulated that of OPN mRNA as compared with the unexposed rats (6.9 +/- 0.22 vs 4.41 +/- 0.29 and 3.26 +/- 0.31 vs 5.28 +/- 0.32, P < 0.01).
CONCLUSIONCGRP can transform the phenotype of CCSMs in diabetic ED rats from contractile to synthetic type.
