Protective effects of notoginsenoside R1 against amyloid-β (1-42) induced mitochondrial apopototic death in SH-SY5Y cells.
- Author:
Tao MA
;
Wen-feng XIN
;
Wen-sheng ZHANG
;
Yong-yan WANG
- Publication Type:Journal Article
- MeSH: Amyloid beta-Peptides; antagonists & inhibitors; Apoptosis; drug effects; Caspases; metabolism; Cell Line, Tumor; Cell Survival; drug effects; Cytoprotection; Ginsenosides; pharmacology; Humans; Mitochondria; drug effects; Peptide Fragments; antagonists & inhibitors
- From: China Journal of Chinese Materia Medica 2015;40(2):303-307
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects and underlying mechanism of notoginsenoside R1 on amyloid-β (1-42) (Aβ(1-42)) induced mitochondrial apoptotic death in SH-SY5Y cells.
METHODCell viability was assayed by MTT, apoptotic rates were analyzed with PI/Annexin V flow cytometry, Bax and Bcl-2 expression were detected with Western blotting, enzymatic activity of caspase-3, caspase-8 and caspase-9 were measured by ELISA assay.
RESULTThe 6.25-100 nmol x L(-1) of notoginsenoside R1 attenuate Aβ(1-42) induced apoptotic death of SH-SY5Y in dose dependent manner. The ratio of Bcl-2/Bax was elevated in SH-SY5Y with notoginsenoside R1 treatment. Caspase-3 and caspase-9 were activated with notoginsenoside R1 treatment while caspase-8 was not affected.
CONCLUSIONNotoginsenoside R1 could protect SH-SY5Y cells from Aβ(1-42) induced apoptosis via mitochondria related apoptotic pathway.