Mutation analysis of the HBV reverse transcriptase in nucleos(t)ide-treated patients with chronic HBV infection
10.3760/cma.j.issn.1003-9279.2012.06.016
- VernacularTitle:核苷(酸)类似物治疗的慢性乙型肝炎患者HBV RT区突变序列分析
- Author:
Xiu-Juan JIAO
1
;
Xun PENG
;
Xiu-Min JIAO
;
Jin-Sheng WANG
;
Xie-Wen SUN
;
Pei-Li ZHAO
;
Shou-Yun WANG
;
Jia-Qun LIU
;
Tong LI
;
Jing-Xian YANG
Author Information
1. 秦皇岛市第三医院
- Keywords:
Hepatitis B virus;
Genotype;
Tolerance;
Nucleosides
- From:
Chinese Journal of Experimental and Clinical Virology
2012;26(6):453-455
- CountryChina
- Language:Chinese
-
Abstract:
Objective To characterize genotypic resistance within HBV RT region in chronic hepatitis B (CHB) patients with nucleos(t) ide analogue (NA) treatment.Methods Serum samples of 229 CHB patients with NA treatment were obtained.Full-length HBV RT sequences were amplified,sequenced and analyzed,on the following NA resistant (NAr) mutations belonging to different NAr pathways.Results Among 229 HBV isolates,14.41％ (33/229) and 85.59％ (196/229) were genotype B and C,respectively;and the patients with HBV genotype C may be more susceptible to develope resistant mutations than patients with HBV genotype B (x2 =2.95,P ＜ 0.05).NAr mutations were detected in 63 CHB patients.Mutations were not found at rtI169,rtT184,rtA194 or rtS202.RtM204 mutations were detected at the highest frequency among 63 mutants (40/63,63.49％) and found to display 11 combination mutation patterns,in which rtM204I were associated with rtL80I/V and rtL180M,and rtM204V were associated with rtL180M,respectively.Conclusions There are complicated mutation patterns in the HBV RT region for chronic hepatitis B (CHB) patients with nucleos(t) ide analogue (NA) treatment.RtM204V/I mutation was the highest.
